Thyroid Hormone Deiodinases and Receptors Are Expressed in Human Endothelial Cells

Publication Type:

Journal Article

Authors:

Laura Sabatino; Silvana Balzan; Valter Lubrano; Giorgio Iervasi

Source:

Biomed Data J., Volume 1, Issue 2, p.19-25 (2015)

Keywords:

deiodinases, endothelial cells, Thyroid hormones

Abstract:

The endothelial system acts as a large endocrine organ in the human body, however, little is still known about the regulative role of THs on endothelial cells. The present study aims to investigate the expression of the TH de­iodinases (D1, D2, D3) and TH receptors (TRα1, TRα2 and TRβ1) in an endothe­lial microvascular cultured cell model (HMEC-1), after stimulation with triiodo­thyronine (T3, 10-100nM), thyroxine (T4, 10-100nM), and reverse T3 (rT3, 1-10nM). The main result of the study is that the TH deiodinases and receptors are expressed in endothelial HMEC-1 cells. These findings might be of signifi­cant clinical relevance, given the important regulatory role of the endothelium as first barrier to the bloodstream.

Full text: 

TH Deiodinases Expressed in Human Endothelial Cells
Polikar R, Burger AG, Scherrer U, Nicod P. The thyroid and the heart. Circulation. 1993;87(5):1435 - 1441.
Bianco AC, Salvatore D, Gereben B, Berry MJ, P Larsen R. Biochemistry, cellular and molecular biology, and physiological roles of the iodothyronine selenodeiodinases. Endocr Rev. 2002;23(1):38-89.
Williams GR. Cloning and Characterization of Two Novel Thyroid Hormone Receptor beta Isoforms. Molecular and Cellular Biology. 2000;20(22):8329 - 8342.
Cheng S-Y, Leonard JL, Davis PJ. Molecular Aspects of Thyroid Hormone Actions. Endocrine Reviews. 2010;31(2):139 - 170.
Berry MJ, Banu L, P. Larsen R. Type I iodothyronine deiodinase is a selenocysteine-containing enzyme. Nature. 1991;349(6308):438 - 440.
Mandel SJ, Berry MJ, Kieffer JD, Harney JW, Warne RL, Larsen PR. Cloning and in vitro expression of the human selenoprotein, type I iodothyronine deiodinase. The Journal of Clinical Endocrinology & Metabolism. 1992;75(4):1133 - 1139.
Visser TJ, Kaptein E, Terpestra OT, Krenning P. Deiodination of Thyroid Hormone by Human Liver. The Journal of Clinical Endocrinology & Metabolism. 1988;67(1):17 - 24.
Tu HM, Kim S-W, Salvatore D, Bartha T, Legradi G, P. Larsen R, Lechan RM. Regional Distribution of Type 2 Thyroxine Deiodinase Messenger Ribonucleic Acid in Rat Hypothalamus and Pituitary and Its Regulation by Thyroid Hormone. Endocrinology. 1997;138(8):3359 - 3368.
Sabatino L, Iervasi G, Ferrazzi P, Francesconi D, Chopra IJ. A study of iodothyronine 5′-monodeiodinase activities in normal and pathological tissues in man and their comparison with activities in rat tissues. Life Sciences. 2000;68(2):191 - 202.
Sabatino L, Chopra IJ, Tanavoli S, Iacconi P, Iervasi G. A Radioimmunoassay for Type I Iodothyronine 5'-Monodeiodinase in Human Tissues. Thyroid. 2001;11(8):733 - 739.
Mori K, Stone S, Braverman LE, Devito WJ. Involvement of tyrosine phosphorylation in the regulation of 5'-deiodinases in FRTL-5 rat thyroid cells and rat astrocytes. Endocrinology. 1996;137(4):1313 - 1318.
Gouveia CHA, Christoffolete MA, Zaitune CR, Dora JMiguel, Harney JW, Maia ALuiza, Bianco AC. Type 2 Iodothyronine Selenodeiodinase Is Expressed throughout the Mouse Skeleton and in the MC3T3-E1 Mouse Osteoblastic Cell Line during Differentiation. Endocrinology. 2005;146(1):195 - 200.
Huang SA, Dorfman DM, Genest DR, Salvatore D, P. Larsen R. Type 3 Iodothyronine Deiodinase Is Highly Expressed in the Human Uteroplacental Unit and in Fetal Epithelium. The Journal of Clinical Endocrinology & Metabolism. 2003;88(3):1384 - 1388.
Huang SA, Fish SA, Dorfman DM, Salvatore D, Kozakewich HPW, Mandel SJ, P. Larsen R. A 21-Year-Old Woman with Consumptive Hypothyroidism due to a Vascular Tumor Expressing Type 3 Iodothyronine Deiodinase. The Journal of Clinical Endocrinology & Metabolism. 2002;87(10):4457 - 4461.
Huang SA, Tu HM, Harney JW, Venihaki M, Butte AJ, Kozakewich HPW, Fishman SJ, P. Larsen R. Severe Hypothyroidism Caused by Type 3 Iodothyronine Deiodinase in Infantile Hemangiomas. New England Journal of Medicine. 2000;343(3):185 - 189.
Sabatino L, Lubrano V, Balzan S, Kusmic C, Del Turco S, Iervasi G. Thyroid hormone deiodinases D1, D2, and D3 are expressed in human endothelial dermal microvascular line: effects of thyroid hormones. Molecular and Cellular Biochemistry. 2015;399(1-2):87 - 94.
Ades EW, Candal FJ, Swerlick RA, George VG, Summers S, Bosse DC, Lawley TJ. HMEC-1: establishment of an immortalized human microvascular endothelial cell line. J Invest Dermatol. 1992;99(6):683-90.
Esfandiari A, Courtin F, Lennon AM, Gavaret JM, Pierre M. Induction of type III deiodinase activity in astroglial cells by thyroid hormones. Endocrinology. 1992;131(4):1682 - 1688.
Pallud S, Ramaugé M, Gavaret J-M, Lennon A-M, Munsch N, Germain DLSt., Pierre M, Courtin F. Regulation of Type 3 Iodothyronine Deiodinase Expression in Cultured Rat Astrocytes: Role of the Erk Cascade. Endocrinology. 1999;140(6):2917 - 2923.
Gouveia CHA, Christoffolete MA, Zaitune CR, Dora JM, Harney JW, Maia AL, Bianco AC. Type 2 iodothyronine selenodeiodinase is express throughout the mouse skeleton and in the MC3T3-E1 mouse osteoblastic cell line during differentiation. Endocrinology. 2005;146(1):195-200.
McAllister RM, Grossenburg VD, Delp MD, Laughlin MH. Effects of hyperthyroidism on vascular contractile and relaxation responses. Am J Physiol. 1998;274(5):E946-53.
Berry MJ, Kates A-L, P. Larsen R. Thyroid Hormone Regulates Type I Deiodinase Messenger RNA in Rat Liver. Molecular Endocrinology. 1990;4(5):743 - 748.
Maia AL, Harney JW, Larsen PR. Pituitary cells respond to thyroid hormone by discrete, gene-specific pathways. Endocrinology. 1995;136(4):1488 - 1494.
Richard K, Hume R, Kaptein ELLEN, Sanders JP, van Toor H, de Herder WW, Hollander JC den, Krenning EP, Visser TJ. Ontogeny of Iodothyronine Deiodinases in Human Liver. The Journal of Clinical Endocrinology & Metabolism. 1998;83(8):2868 - 2874.
Maia AL, Goemann IM, Meyer ELS, Wajner SM. Type 1 iodothyronine deiodinase in human physiology and disease: Deiodinases: the balance of thyroid hormone. Journal of Endocrinology. 2011;209(3):283 - 297.
Salvatore D, Simonides WS, Dentice M, Zavacki AMarie, P. Larsen R. Thyroid hormones and skeletal muscle—new insights and potential implications. Nature Reviews Endocrinology. 2013;10(4):206 - 214.
Germain DLSt. The Effects and Interactions of Substrates, Inhibitors, and the Cellular Thiol- Disulfide Balance on the Regulation of Type II Iodothyronine 5′-Deiodinase*. Endocrinology. 1988;122(5):1860 - 1868.
Burmeister LA, Pachucki J, Germain DLSt.. Thyroid Hormones Inhibit Type 2 Iodothyronine Deiodinase in the Rat Cerebral Cortex by Both Pre- and Posttranslational Mechanisms. Endocrinology. 1997;138(12):5231 - 5237.
Gereben B, Goncalves C, Harney JW, P. Larsen R, Bianco AC. Selective Proteolysis of Human Type 2 Deiodinase: A Novel Ubiquitin-Proteasomal Mediated Mechanism for Regulation of Hormone Activation. Molecular Endocrinology. 2000;14(11):1697 - 1708.
Gereben B, Kollár A, Harney JW, P. Larsen R. The mRNA Structure Has Potent Regulatory Effects on Type 2 Iodothyronine Deiodinase Expression. Molecular Endocrinology. 2002;16(7):1667 - 1679.
Martinez-deMena R, Hernández A, Obregón M-J. Triiodothyronine is required for the stimulation of type II 5′-deiodinase mRNA in rat brown adipocytes. American Journal of Physiology - Endocrinology And Metabolism. 2002;282(5):E1119 - E1127.
Steinsapir J, Harney J, Larsen PR. Type 2 iodothyronine deiodinase in rat pituitary tumor cells is inactivated in proteasomes. Journal of Clinical Investigation. 1998;102(11):1895 - 1899.
St Germain DL, Schwartzman RA, Croteau W, Kanamori A, Wang Z, Brown DD, Galton VA. A thyroid hormone-regulated gene in Xenopus laevis encodes a type III iodothyronine 5-deiodinase. Proc Natl Acad Sci U S A. 1994;91(16):7767-71.
Van der Geyten S, Buys N, Sanders JP, Decuypere E, Visser TJ, Kühn ER, Darras VM. Acute pretranslational regulation of type III iodothyronine deiodinase by growth hormone and dexamethasone in chicken embryos. Molecular and Cellular Endocrinology. 1999;147(1-2):49 - 56.
Kinugawa K, Yonekura K, Ribeiro RCJ, Eto Y, Aoyagi T, Baxter JD, Camacho SA, Bristow MR, Long CS, Simpson PC. Regulation of Thyroid Hormone Receptor Isoforms in Physiological and Pathological Cardiac Hypertrophy. Circulation Research. 2001;89(7):591 - 598.
Balzan S, Del Carratore R, Nicolini G, Beffy P, Lubrano V, Forini F, Iervasi G. Proangiogenic Effect of TSH in Human Microvascular Endothelial Cells through Its Membrane Receptor. The Journal of Clinical Endocrinology & Metabolism. 2012;97(5):1763 - 1770.
Ojamaa K, Klemperer JD, Klein I. Acute Effects of Thyroid Hormone on Vascular Smooth Muscle. Thyroid. 1996;6(5):505 - 512.
Savinova OV, Liu Y, Aasen GA, Mao K, Weltman NY, Nedich BL, Liang Q, A. Gerdes M. Thyroid Hormone Promotes Remodeling of Coronary Resistance Vessels. Anversa P. PLoS ONE. 2011;6(9):e25054.
Carrillo-Sepulveda MA, Ceravolo GS, Fortes ZB, Carvalho MH, Tostes RC, Laurindo FR, Webb RC, Barreto-Chaves MLM. Thyroid hormone stimulates NO production via activation of the PI3K/Akt pathway in vascular myocytes. Cardiovascular Research. 2010;85(3):560 - 570.
Napoli R, Guardasole V, Angelini V, Zarra E, Terracciano D, D’Anna C, Matarazzo M, Oliviero U, Macchia V, Saccà L. Acute Effects of Triiodothyronine on Endothelial Function in Human Subjects. The Journal of Clinical Endocrinology & Metabolism. 2007;92(1):250 - 254.
National Library of Medicine (brackets, no "et al."): Sabatino L, Balzan S, Lubrano V, Iervasi G. Thyroid Hormone Deiodinases and Receptors Are Expressed in Human Endothelial Cells. Biomed Data J. 2015;1(2):19-25.
National Library of Medicine (grant proposals with PMCID/PMID): Sabatino L, Balzan S, Lubrano V, Iervasi G. Thyroid Hormone Deiodinases and Receptors Are Expressed in Human Endothelial Cells. Biomed Data J. 2015;1(2):19-25.
National Library of Medicine (NLM) - Grant with PMID: Sabatino L, Balzan S, Lubrano V, Iervasi G. Thyroid Hormone Deiodinases and Receptors Are Expressed in Human Endothelial Cells. Biomed Data J. 2015;1(2):19-25.
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